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Spiroplasma fibrils.

D L Williamson, P R Brink, G W Zieve

    Israel Journal of Medical Sciences
    |September 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Spiroplasmas, wall-less helical microorganisms, maintain their shape through internal microfibrils composed of a 55,000-dalton protein. These fibrils are a common feature across Spiroplasma species, aiding in cellular structure.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Biophysics

    Background:

    • Spiroplasmas are wall-less microorganisms with a distinct helical morphology.
    • Understanding the structural basis of this morphology is a fundamental biological question.

    Purpose of the Study:

    • To investigate the internal structures responsible for maintaining spiroplasma morphology.
    • To characterize the protein composition and organization of these internal structures.

    Main Methods:

    • Detergent-based demembranation of spiroplasmas.
    • Transmission electron microscopy (TEM) for visualizing microfibrils.
    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and protein blotting for protein analysis.
    • Density gradient centrifugation for fibril purification.

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    Main Results:

    • Demembranation revealed numerous striated microfibrils (4 nm width, 9 nm repeat) within spiroplasmas.
    • These fibrils are composed of a 55,000-dalton protein, conserved across tested spiroplasma serogroups.
    • Fibrils are present in both helical and nonhelical spiroplasma species.

    Conclusions:

    • Internal microfibrils are crucial for maintaining spiroplasma morphology.
    • A conserved 55,000-dalton protein forms these essential structural elements.
    • Further research is needed to fully elucidate fibril organization within the spiroplasma cell.