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[Spectrophotometric evaluation of hemolysis tests]
Zeitschrift Fur Experimentelle Chirurgie, Transplantation, Und Kunstliche Organe : Organ Der Sektion Experimentelle Chirurgie Der Gesellschaft Fur Chirurgie Der DDR
|January 1, 1984
Summary
A new spectrophotometric method accurately measures the hemolysis degree of PVC-biomaterials. This method helps assess biomaterials for hemolytically acting substances, with higher extraction temperatures recommended for detection.
Area of Science:
- Biomaterials Science
- Analytical Chemistry
- Spectrophotometry
Background:
- Assessing the biocompatibility of Polyvinyl Chloride (PVC) biomaterials is crucial.
- Hemolysis, the rupture of red blood cells, is a key indicator of adverse reactions to biomaterials.
Purpose of the Study:
- To present a quantitative spectrophotometric method for measuring the hemolysis degree of PVC-biomaterials.
- To enhance the physiological valuation of biomaterials regarding hemolytically acting substances.
Main Methods:
- Quantitative spectrophotometric analysis using potassium hexacyanoferrate (III) and potassium cyanide.
- Extraction of biomaterial components at 70°C and 121°C.
- Analysis of extracts using UV and IR spectroscopy.
Main Results:
- The developed method provides a more exact physiological valuation of biomaterials concerning hemolytically acting substances.
- UV and IR spectrograms revealed unidentified substances in both 70°C and 121°C extracts.
- No specific biological effects were attributed to the identified substances.
Conclusions:
- Further studies are required to establish the maximum permissible degree of hemolysis for biomaterials.
- Extraction at 121°C is recommended for effectively detecting hemolytically acting substances in biomaterials.