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A method for freezing bovine lymphocytes.

R A Oliver, A J Springbett, R L Spooner

    Animal Blood Groups and Biochemical Genetics
    |January 1, 1984
    PubMed
    Summary
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    This study details a simple two-stage method for preserving bovine lymphocytes. Optimal cryopreservation involved freezing at -29 to -31°C with high dimethylsulphoxide (DMSO) concentrations (17.5-20%).

    Area of Science:

    • Veterinary Immunology
    • Cryobiology
    • Cell Preservation

    Background:

    • Bovine lymphocytes are crucial for immunological studies and vaccine development.
    • Effective cryopreservation techniques are essential for long-term storage and transport of lymphocytes.
    • Existing methods often face challenges with viability and recovery rates.

    Purpose of the Study:

    • To develop and optimize a simple two-stage cryopreservation technique for bovine lymphocytes.
    • To determine the optimal freezing temperature and dimethylsulphoxide (DMSO) concentration for preserving bovine lymphocytes.
    • To evaluate the efficacy of the proposed method compared to existing protocols.

    Main Methods:

    • Utilized a simple two-stage freezing technique for bovine lymphocytes.

    Related Experiment Videos

  • Investigated a range of freezing temperatures, focusing on -29°C to -31°C.
  • Assessed varying concentrations of dimethylsulphoxide (DMSO) as a cryoprotective agent, specifically between 17.5% and 20%.

    • Main Results:

    • Identified an optimal freezing temperature range of -29°C to -31°C for bovine lymphocyte preservation.
    • Determined that optimal cryoprotection was achieved with high dimethylsulphoxide (DMSO) concentrations, ranging from 17.5% to 20%.
    • Observed that these DMSO concentrations are significantly higher than those typically reported in other studies.

    Conclusions:

    • A simple two-stage technique effectively preserves bovine lymphocytes.
    • High concentrations of DMSO (17.5-20%) combined with specific freezing temperatures (-29°C to -31°C) are optimal for bovine lymphocyte cryopreservation.
    • This method offers a potentially improved approach for preserving bovine lymphocytes for research and clinical applications.