Estimation of thromboxane B2 in the clotting human whole blood by gas chromatography

In recent years, the interest within platelet reactions has been focused increasingly on thromboxane (TX) A2 formation. Thrombin formed in clotting blood stimulates platelets in their natural environment to produce TXA2. For estimation of TXB2 - the hydrolysis product of TXA2 - in serum of human blood clotted in vitro we used an internal standardization with 2,3,-dinor-TXB2 and the electron capture gas chromatography of the methoxime pentafluorobenzyl ester trimethylsilyl ether derivatives. The reliability of method was characterized by means of coefficients of variation at different stages of assay. Influences of various venous blood sampling and incubation conditions, and of addition of exogenous thrombin to native or citrated blood were investigated.