[Study of bone tissue structure using polarized Raman spectra]

Polarized Raman spectra of bone tissue were obtained as well as of collagen fibres of the mouse tail tendon and oxyapatite crystals. It is shown that polarized Raman spectra permit to obtain information on spatial orientation of collagen fibres and oxyapatite microcrystals in various parts of the bone tissue. A laser Raman microprobe "Mole" was used to take Raman spectra measurements from the area of several square micrones. It is shown that in the diaphis osteon of human femur the collagen fibres and crystallographic axis C of oxyapatite crystals are directed along the long axis of the bone in the direction of the main mechanical loads on it.