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Cells transformed by certain strains of Moloney sarcoma virus contain murine p60

W G Robey, M K Oskarsson, G F Vande Woude

    Cell
    |January 1, 1977
    PubMed

    Insights

    The feline leukemia virus pseudotype of Moloney sarcoma virus produces a p60 polyprotein, not murine p30, in transformed cells. This p60 protein is specified by the virus genome and is inefficiently cleaved before virus release.

    Area of Science:

    • Retroviral research
    • Molecular virology
    • Oncogenesis

    Background:

    • Murine p30 was previously identified as a constituent of the feline leukemia virus pseudotype of Moloney sarcoma virus [m1MSV(FeLV)].
    • The antigenic reactivity of S + L- cells was attributed to murine p30.

    Purpose of the Study:

    • To investigate the polypeptide composition of Moloney sarcoma virus (MSV)-transformed cells.
    • To determine if the p60 polyprotein is specified by the MSV genome.
    • To elucidate the processing and cleavage of p60 in relation to p30 production.

    Main Methods:

    • Detection of polypeptides in MSV-transformed cells from five mammalian species using immunoprecipitation.
    • Pulse-chase studies to analyze intracellular polypeptide synthesis and processing.
    • Comparison of polypeptide expression across different MSV strains (m1MSV, m3MSV, HT-1 MSV).

    Main Results:

    • The p60 polyprotein, not murine p30, was detected in m1MSV-transformed cells across multiple species.
    • p60 is specified by the m1MSV genome and is the largest polypeptide synthesized during pulse labeling.
    • Intracellular p60 showed inefficient cleavage into smaller polypeptides like p30 during chase periods, with p30 appearing extracellularly before virus release.
    • Distinct polypeptide profiles (p60, p70, or none) were observed in m3MSV and HT-1 MSV-transformed cells, suggesting genetic differences.

    Conclusions:

    • The p60 polyprotein is the primary translation product specified by the m1MSV genome.
    • Cleavage of p60 to p30 is an inefficient intracellular process occurring late, likely just before virus egress.
    • Differences in polypeptide expression among MSV strains indicate variations in their genetic makeup and oncogenic potential.

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