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[An economical method for research on serum triacylglycerols in mass screening]
Abstract:
A method with thin layer chromatography on alufoil of the firm "Merck" was developed for the needs of mass screening in the studies on lipid metabolism. Serum lipids extract is divided at a liquid phase benzine-diethyl ether 4:1. After carbonization, lipid spots are observed. The fourth of them is of TAG. The intensity of the spot is compared with the standard triolein. In this way the sera with hypertriglyceridemia are separated and only a small part of the mass screening (5-8%) are further studied by enzyme or other methods. In this way time and means are largely saved. Because of the small quantity of serum necessary for the investigations, the method is recommended for the pediatric practice as well.