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A sensitive double isotopic, chromatographic method for the determination of histamine. I: Studies with authentic
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|October 1, 1984
Abstract:
This paper presents a method for the determination of histamine using an internal standard of 14C-labelled histamine, which together with an unknown sample of unlabelled histamine is dansylated with 3H-labelled dansyl chloride. The products of this dansylation are then separated on thin-layer-chromatographic plates coated with micropolyamide. The spot of dansylhistamine is scraped off and the content of histamine in the unknown sample is calculated from the 3H/14C-ratio in the spot.