Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amount of nickel applied with a standard patch test.

T Fischer, H Maibach

    Contact Dermatitis
    |November 1, 1984
    PubMed
    Summary

    Nickel sulphate concentrations in patch tests vary significantly. Standard patch tests can apply up to six times more nickel than intended, impacting nickel allergy diagnosis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pathophysiology and management of sensitive skin: position paper from the special interest group on sensitive skin of the International Forum for the Study of Itch (IFSI).

    Journal of the European Academy of Dermatology and Venereology : JEADV·2019
    Same author

    Comparing touch senses of naïve and expert panels through treated hair swatches: which associated wordings correlate with hair physical properties?

    International journal of cosmetic science·2017
    Same author

    Skin absorption through atopic dermatitis skin: a systematic review.

    The British journal of dermatology·2016
    Same author

    Physiological effect of olfactory stimuli inhalation in humans: an overview.

    International journal of cosmetic science·2013
    Same author

    Metabolic enzyme activities and drug excretion in the small intestine and in the liver in the rat.

    Acta physiologica Hungarica·2013
    Same author

    Characterization of focal breast lesions by means of elastography.

    RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin·2013

    Area of Science:

    • Dermatology
    • Allergology
    • Analytical Chemistry

    Background:

    • Nickel sulphate is a common allergen.
    • Patch testing is a standard diagnostic method for nickel allergy.
    • Accurate antigen concentration is crucial for reliable patch test results.

    Purpose of the Study:

    • To determine the actual concentration of nickel sulphate in commercially available patch test antigens.
    • To assess the variability in nickel sulphate concentration and its impact on patch testing.

    Main Methods:

    • Analysis of nickel sulphate concentration in commercially sourced patch test samples (9-16 mg).
    • Utilized two different manufacturers for 2.5% nickel sulphate in petrolatum.
    • Included one manufacturer for 5.0% nickel sulphate in petrolatum.

    Main Results:

    • The concentration of 2.5% nickel sulphate samples ranged from 0.7% to 1.1% (+/- 2 SD).
    • The concentration of 5.0% nickel sulphate samples showed a range of +/- 0.5%.
    • The volume of patch test material applied using the Finn chamber technique varied from 9 to 50 microliters, exceeding the standard 12-18 microliters.

    Conclusions:

    • Significant variability exists in the actual nickel sulphate concentration of commercial patch test antigens.
    • The amount of nickel applied during standard patch tests can vary by at least six-fold.
    • This variability may affect the accuracy and reliability of nickel allergy diagnosis through patch testing.

    Related Experiment Videos