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[Protective effect of alpha-tocopherol on the hydroxylating system in liver endoplasmic reticulum membranes against
Abstract:
Effect of hyperbaric oxygenation (oxygen pressure 3039 hPa, exposition 3 hrs) on activity of the hydroxylating system and lipid peroxidation was studied in membranes of rat liver endoplasmic reticulum. Within the first 3 hrs after hyperbaric oxygenation the rates of aniline n-hydroxylation, amidopyrine N-demethylation, NADPH oxidation and the cytochrome P-450 level were decreased, while the rates of ascorbate- and NADPH-dependent lipid peroxidation were increased. Oil emulsion of alpha-tocopherol, administered per os into rats at a dose of 50 mg/kg every 12 hrs within 2 days before hyperbaric oxygenation, prevented the posthyperoxic deterioration of xenobiotic metabolism and the intensification of lipid peroxidation in liver microsomes.