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Effect of surfactants on the separation of proteins by reversed-phase high-performance liquid chromatography. I.
Journal of Chromatography
|December 28, 1984
Abstract:
The retention of a number of proteins on diphenyl-bonded silica coated with non-ionic surfactants (Tween) was investigated. It was found that a hydrophilic surface is formed by the interaction of Tween with the diphenyl groups on the packing surface. The hydrophilic stationary phase generated can be used for the size-exclusion chromatography of proteins.