Skin determination of the 8-methoxypsoralen concentration in rats

This paper describes a method for determining the amount of 8-methoxypsoralen in rat skin tissue by means of high performance liquid chromatography. The 8-methoxypsoralen was extracted with an organic solvent out of homogenized skin tissue. Following evaporation of the solvent, the extracts could be injected directly into the column without further purification. The procedure is relatively simple.