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Effect of intravenously administered iodipamide ethyl ester particles on rat liver morphology
Abstract:
The effect of intravenously injected iodipamide ethyl ester (IDE) particles (150 mgI/kg) on the rat liver was studied by light and electron microscopy. IDE particles were phagocytized by Kupffer cells within a few minutes postinjection. After 4 hours particles began to dissolve in the Kupffer cells causing transient morphologic alterations resembling hydropic degeneration. The number of Kupffer cells seemed, however, to be unchanged and were ultrastructurally normal by 17 days postinjection. Hepatocytes contained large lipid droplets from one to four days after IDE injection. No changes were found in the hepatocytes from one week to one year after IDE injection. The fat-storing cells and endothelial cells remained ultrastructurally normal throughout the study.
Insights
Iodipamide ethyl ester (IDE) particles are quickly absorbed by rat liver Kupffer cells, causing temporary cell changes. Hepatocytes show lipid droplets initially but recover, with all liver cells returning to normal within weeks.
Area of Science:
- Hepatology
- Toxicology
- Cell Biology
Background:
- Iodipamide ethyl ester (IDE) is an iodinated contrast agent.
- Understanding the liver's response to contrast agents is crucial for patient safety.
Purpose of the Study:
- To investigate the short- and long-term effects of iodipamide ethyl ester (IDE) particles on rat liver cells.
- To assess the ultrastructural changes in Kupffer cells, hepatocytes, fat-storing cells, and endothelial cells following IDE administration.
Main Methods:
- Light and electron microscopy were used to examine liver tissue.
- Rats were intravenously injected with iodipamide ethyl ester (IDE) particles at a dose of 150 mgI/kg.
- Liver tissues were analyzed at various time points post-injection.
Main Results:
- IDE particles were rapidly phagocytized by Kupffer cells.
- Transient morphologic alterations, resembling hydropic degeneration, were observed in Kupffer cells within hours.
- Hepatocytes exhibited large lipid droplets from 1 to 4 days post-injection.
- Kupffer cells, fat-storing cells, and endothelial cells showed no significant ultrastructural changes by 17 days or long-term.
Conclusions:
- The rat liver demonstrates a transient response to iodipamide ethyl ester (IDE) particles, primarily affecting Kupffer cells and hepatocytes initially.
- Liver cells, including Kupffer cells and hepatocytes, exhibit a remarkable capacity for recovery, returning to normal ultrastructure within weeks.
- IDE appears to be well-tolerated by the rat liver with no persistent adverse effects observed in the studied cell types.