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Computational analysis of models for cotransport.

D A Harrison, G W Rowe, C J Lumsden

    Biochimica Et Biophysica Acta
    |July 11, 1984
    PubMed
    Summary
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    This study numerically fits theoretical models to Na+-D-glucose cotransport data. The Glide model shows applicability, but complete parameterization is hindered by potential temperature heterogeneity.

    Area of Science:

    • Biophysics
    • Biochemical kinetics
    • Membrane transport

    Background:

    • Understanding substrate interaction order with cotransport carriers is crucial for elucidating transport mechanisms.
    • The Na+-D-glucose cotransporter is a model system for studying secondary active transport.

    Purpose of the Study:

    • To investigate the order of substrate interaction with a cotransport carrier using theoretical models and empirical data.
    • To compare Random, Ordered mirror, and Ordered glide models for the Na+-D-glucose pathway.
    • To estimate rate constants for the kinetic steps in the Na+-D-glucose cotransport mechanism.

    Main Methods:

    • Numerical fitting of theoretical models to empirical data.
    • Utilizing a least-squares minimization routine.

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  • Analyzing tracer flux data under equilibrium exchange conditions.

    • Main Results:

    • Both Random and Glide models demonstrated applicability to the experimental observations.
    • The Glide model allowed for estimation of relative values of individual rate constants.
    • Complete parameterization of the Glide model was not attained, possibly due to experimental temperature heterogeneity.

    Conclusions:

    • Model discrimination is achievable through parameterization, even with unknown kinetic mechanisms.
    • Estimating individual rate constants aids in understanding carrier kinetic fine structure.
    • Three solutions for the Glide model were presented, each fitting a subset of the data.