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Mononuclear cell (MNC) collection by continuous-flow centrifugation (CFC).

J P Hester, R M Kellogg, E J Freireich

    Journal of Clinical Apheresis
    |January 1, 1983
    PubMed
    Summary
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    Continuous-flow centrifugation effectively separates mononuclear cells, with higher G-forces increasing yield but risking platelet depletion. Optimal settings balance cell collection efficiency with blood component preservation for research or therapeutic applications.

    Area of Science:

    • Hematology
    • Cellular Biology
    • Biomedical Engineering

    Background:

    • Mononuclear cell separation is crucial for various research and clinical applications.
    • Continuous-flow centrifugation offers a method for large-scale cell collection.
    • Understanding the impact of centrifugation parameters on cell yield and quality is essential.

    Purpose of the Study:

    • To investigate the relationship between centrifugal forces and mononuclear cell recovery.
    • To determine the effect of processing volume on cell yield.
    • To assess the impact of continuous-flow centrifugation on blood components, particularly platelets.

    Main Methods:

    • Continuous-flow centrifugation was employed to process varying volumes of peripheral blood.
    • Centrifugal forces (G-forces) were adjusted to evaluate their effect on cell separation.

    Related Experiment Videos

  • Parameters such as citrate infusion rates and ionized calcium levels were monitored.

    • Main Results:

    • Lower G-forces cleared 70-80% of mononuclear cells, while higher G-forces achieved 95-100% clearance.
    • Increased G-forces led to significant platelet depletion.
    • Harvesting 2-3 liters of blood yielded approximately 3 billion cells, increasing to 9 billion with 6 liters processed.
    • Mononuclear cells were collected in large quantities from normal donors without substantial depletion.

    Conclusions:

    • Continuous-flow centrifugation is an effective method for mononuclear cell collection.
    • Optimizing G-forces is critical to maximize mononuclear cell yield while minimizing platelet loss.
    • The volume of blood processed directly correlates with the total number of mononuclear cells harvested.