S-(1,2-dicarboxyethyl)glutathione and S-(1,2-dicarboxyethyl)L-cysteine in lens

S-(1,2-Dicarboxyethyl)glutathione and S-(1,2-dicarboxyethyl)L-cysteine were determined by high performance liquid chromatography after reaction with 2,4-dinitrofluorobenzene. By this method the former could be determined in the range 4.05 mumol/l-815 mumol/l, and the latter in the range 1.45 mumol/l-1.45 mmol/l. The recovery from cattle lens homogenate was 90.0 +/- 3.2% for S-(1,2-dicarboxyethyl)glutathione and 95.3 +/- 3.1% for S-(1,2-dicarboxyethyl)L-cysteine. Using this method S-(1,2-dicarboxyethyl)-glutathione and S-(1,2-dicarboxyethyl)L-cysteine were determined in lenses of several vertebrates and in rat lens during cataract formation by galactose.