Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The structure of histargin.

K Ogawa, H Naganawa, H Iinuma

    The Journal of Antibiotics
    |September 1, 1984
    PubMed
    Summary

    Researchers identified the chemical structure of histargin, a carboxypeptidase B inhibitor from Streptomyces roseoviridis. This discovery aids in understanding enzyme inhibition and developing new therapeutic agents.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Acceleration of Positive Muons by a Radio-Frequency Cavity.

    Physical review letters·2025
    Same author

    Erratum: Evolution of π^{0} Suppression in Au+Au Collisions from sqrt[s_{NN}]=39 to 200 GeV [Phys. Rev. Lett. 109, 152301 (2012)].

    Physical review letters·2020
    Same author

    Beam Energy and Centrality Dependence of Direct-Photon Emission from Ultrarelativistic Heavy-Ion Collisions.

    Physical review letters·2019
    Same author

    Measurements of Elliptic and Triangular Flow in High-Multiplicity 3He+Au Collisions at √(s(NN))=200 GeV.

    Physical review letters·2015
    Same author

    Exosomal microRNA in serum is a novel biomarker of recurrence in human colorectal cancer.

    British journal of cancer·2015
    Same author

    Measurement of Long-Range Angular Correlation and Quadrupole Anisotropy of Pions and (Anti)Protons in Central d+Au Collisions at sqrt[s_{NN}]=200 GeV.

    Physical review letters·2015

    Area of Science:

    • Biochemistry
    • Microbiology
    • Organic Chemistry

    Background:

    • Carboxypeptidase B is a key enzyme in various physiological processes.
    • Histargin is a novel inhibitor of carboxypeptidase B.
    • Streptomyces roseoviridis is a known producer of bioactive compounds.

    Purpose of the Study:

    • To elucidate the precise chemical structure of histargin.
    • To confirm the structure through chemical synthesis.
    • To provide a basis for understanding histargin's inhibitory mechanism.

    Main Methods:

    • Spectral analysis (e.g., NMR, Mass Spectrometry) was employed.
    • Chemical synthesis was performed to validate the proposed structure.
    • Isolation and purification from Streptomyces roseoviridis MF118-A5 strain.

    Main Results:

    • The structure of histargin was determined to be N-[(S)-1-carboxy-4-guanidinobutyl]-N'-[(S)-1-carboxy-2-(imidazol-4 -yl) ethyl]ethylenediamine.
    • Spectral data confirmed the proposed molecular structure.
    • Chemical synthesis successfully yielded a compound identical to natural histargin.

    Conclusions:

    • The definitive structure of histargin, a carboxypeptidase B inhibitor, has been established.
    • This structural information is crucial for future research on enzyme inhibition.
    • The findings pave the way for the development of novel therapeutic strategies targeting carboxypeptidase B.

    Related Experiment Videos