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Headgroup interactions in mixed phospholipid bilayers.

F Sixl, A Watts

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1983
    PubMed
    Summary
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    Deuterium NMR reveals how phospholipid headgroups interact within lipid bilayers. Charged lipids significantly alter the motion of zwitterionic phospholipid headgroups, impacting membrane surface structure and water interactions.

    Area of Science:

    • Biophysics
    • Membrane Biophysics
    • Nuclear Magnetic Resonance Spectroscopy

    Background:

    • Lipid bilayers are fundamental to cell membranes.
    • Understanding phospholipid headgroup interactions is crucial for membrane function.
    • Deuterium NMR (2H NMR) is a powerful tool for studying molecular dynamics in membranes.

    Purpose of the Study:

    • To investigate phospholipid headgroup interactions in mixed bilayers using 2H NMR.
    • To quantify the effect of charged lipids on the dynamics of zwitterionic phospholipids.
    • To explore changes in membrane surface water structure due to lipid composition.

    Main Methods:

    • Preparation of aqueous dispersions of specifically deuterated phospholipids.
    • Acquisition and analysis of 2H NMR powder spectra.

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  • Measurement of quadrupole splittings to determine headgroup motion.

    • Main Results:

    • 2H NMR spectra provided insights into headgroup dynamics in lipid bilayers.
    • Mixed bilayers showed altered quadrupole splittings, indicating lipid-lipid interactions.
    • Charged lipids (phosphatidylserine, phosphatidylglycerol) strongly affected choline and ethanolamine headgroup motion in zwitterionic lipids.
    • Zwitterionic lipid mixtures (phosphatidylcholine/phosphatidylethanolamine) also exhibited altered headgroup motion.

    Conclusions:

    • Phospholipid headgroup interactions significantly influence membrane surface dynamics.
    • The presence of charged lipids induces substantial changes in zwitterionic lipid headgroup mobility.
    • These changes suggest perturbations in hydrogen bonding and/or membrane surface water structure.