Glutathione mediated lysis of HL-60 cells

Various concentrations of glutathione (GSH), between 50-2000 micrograms/ml, were shown to retard the proliferation of HL-60 cells, with the optimum level being 200 micrograms/ml. Using electronic cell volume, GSH was shown to increase the percentage of small size cells and debris, and decrease the population cell volume and the percentage of large size cells. This data combined with chromium-51 release, trypan blue exclusion, and morphology data suggested that GSH was lysing HL-60 cells. Catalase eliminated the GSH stimulated cell lysis suggesting a H2O2 mediated mechanism was involved.