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Collagen fibril formation during embryogenesis.

R Fleischmajer, B R Olsen, R Timpl

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1983
    PubMed
    Summary
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    The amino propeptide of type I collagen is crucial for forming thin collagen fibrils during embryonic development. Its removal is associated with the growth of thicker collagen fibrils in chicken skin.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Developmental Biology

    Background:

    • Collagen is a vital structural protein essential for connective tissues.
    • Collagen fibrillogenesis, the process of forming collagen fibrils, is complex and not fully understood.
    • The role of collagen propeptides in fibril formation requires further investigation.

    Purpose of the Study:

    • To investigate the role of amino and carboxyl propeptides of type I collagen in collagen fibrillogenesis.
    • To determine the localization and function of pN-collagen during fibril formation.
    • To elucidate the mechanisms underlying collagen fibril thickening.

    Main Methods:

    • Indirect immunoelectron microscopy using ferritin-labeled antibodies.
    • Labeling of chicken embryo and adult skin with antibodies against type I and type III collagen propeptides.

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  • Extraction and identification of pN-collagen chains via gel electrophoresis and immunoblotting.

    • Main Results:

    • Thin collagen fibril formation involves the polymerization of pN-collagen.
    • Amino propeptides are absent in collagen fibrils thicker than 35-40 nm.
    • Carboxyl propeptides are associated with thin fibrils but lack periodic arrangement.
    • Type III collagen amino propeptides show periodic labeling on fibrils of 20-40 nm diameter.
    • Significant pN-collagen amounts were found in embryonic chicken skin.

    Conclusions:

    • The amino propeptide plays a critical role in initiating collagen fibrillogenesis in embryonic skin.
    • Fibril thickening may occur through lateral aggregation or fusion of thinner fibrils.
    • pN-collagen is a key component in the early stages of collagen fibril formation.