Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Adenosine deaminase activity in hemoblastoses].

W Krüger, G Müller, H Storch

    Archiv Fur Geschwulstforschung
    |January 1, 1983
    PubMed
    Summary

    Adenosine deaminase (ADA) levels in blood cells differ across various blood cancers. Decreased ADA activity is seen in chronic lymphocytic leukemia, Hodgkin

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The cAMP-binding ectoprotein from Saccharomyces cerevisiae is membrane-anchored by glycosyl-phosphatidylinositol.

    The Journal of biological chemistry·1992
    Same author

    Conformation/activity studies of rationally designed potent anti-adhesive RGD peptides.

    European journal of biochemistry·1992
    Same author

    Pituitary factors in blood plasma are necessary for smooth muscle cell proliferation in response to injury in vivo.

    Arteriosclerosis and thrombosis : a journal of vascular biology·1992
    Same author

    [Avoidable perinatal mortality. Attempted evaluation and significance].

    Presse medicale (Paris, France : 1983)·1992
    Same author

    [Response to intermittent photic stimulation in patients with suspected dementia in comparison with healthy aging].

    Der Nervenarzt·1992
    Same author

    [Laser in surgery of vascular anastomoses].

    Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...·1992

    Area of Science:

    • Biochemistry
    • Hematology
    • Immunology

    Background:

    • Adenosine deaminase (ADA) is a key enzyme in purine metabolism.
    • Altered ADA activity has been implicated in various diseases, including hematological malignancies.
    • Understanding ADA levels can provide insights into disease mechanisms and immune function.

    Purpose of the Study:

    • To investigate adenosine deaminase activity in patients with diverse hematological malignancies.
    • To correlate ADA levels with specific types of leukemia and lymphoma.
    • To explore the relationship between ADA activity and immunological status in cancer patients.

    Main Methods:

    • Enzyme activity assay of adenosine deaminase using adenosine as substrate.
    • Measurement of released ammonia to quantify ADA activity.
    • Analysis of blood samples (lymphocytes, erythrocytes, plasma) from 73 patients and healthy controls.

    Main Results:

    • Significantly decreased ADA levels were observed in lymphocytes and erythrocytes of patients with chronic lymphocytic leukemia, Hodgkin's disease, and multiple myeloma.
    • Elevated ADA levels were found in lymphocytes or blast cells of patients with myeloid leukemia, blast crisis, and acute leukemias.
    • A positive correlation was found between blast cell count in peripheral blood and ADA activity in the mononuclear cell fraction.

    Conclusions:

    • Reduced ADA activity in certain hematological malignancies may be linked to impaired immune function.
    • Increased ADA levels in other leukemias suggest heightened purine metabolism in blast cells.
    • ADA activity serves as a potential biomarker for distinguishing and understanding different hematological cancers.

    Related Experiment Videos