Hexosaminidase A in amniotic fluid of Tay-Sachs fetuses

Hexosaminidase A is present in relatively low concentrations in cell-free amniotic fluids from pregnancies with Tay-Sachs fetuses. This isoenzyme was determined by an immunological procedure, radial immunodiffusion, by which hexosaminidase A can be directly and specifically detected, even in the presence of excess amounts of hexosaminidase B. No hexosaminidase A could be detected by the same procedure in Tay-Sachs fetal tissues, implying that this isoenzyme in the amniotic fluid originates from the mother.