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Nickel-sequestering renal glycoprotein.

A W Abdulwajid, B Sarkar

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1983
    PubMed
    Summary
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    Researchers identified a novel low molecular weight glycoprotein in rat kidneys that binds nickel. This protein, potentially part of the renal basement membrane, may play a role in handling and excreting toxic metals.

    Area of Science:

    • Biochemistry
    • Toxicology
    • Renal Physiology

    Background:

    • Nickel is a toxic metal that accumulates in the kidneys.
    • Understanding the mechanisms of nickel accumulation and excretion is crucial for renal health.
    • Low molecular weight proteins can bind and transport metals within cells.

    Purpose of the Study:

    • To isolate and characterize the protein responsible for nickel binding in rat kidneys.
    • To investigate the nature and potential function of this nickel-binding protein.
    • To determine if the protein's nickel-binding capacity is affected by actinomycin D.

    Main Methods:

    • Intraperitoneal administration of radioactive 63NiCl2 to rats.
    • Isolation and purification of the nickel-binding protein from kidney cytosol.

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  • Characterization using polyacrylamide gel electrophoresis, amino acid analysis, and carbohydrate analysis.
  • In vitro nickel addition experiments and in vivo actinomycin D administration.

    • Main Results:

    • A homogeneous low molecular weight (15,000-16,000 Da) glycoprotein was isolated.
    • The glycoprotein is rich in glycine and proline, with high mannose-type carbohydrates.
    • Nickel was found bound to this protein in kidney cytosol.
    • Actinomycin D did not affect the protein's nickel-binding capacity, suggesting it is non-inducible.

    Conclusions:

    • The identified glycoprotein exhibits characteristics of renal basement membrane components.
    • This protein may be involved in nickel detoxification and excretion.
    • It potentially binds other toxic metals, contributing to natural metal handling processes in the kidney.