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Related Experiment Videos

Specific DNA sequence amplification in human neuroblastoma cells.

K T Montgomery, J L Biedler, B A Spengler

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1983
    PubMed
    Summary

    Specific DNA sequences are amplified in most human neuroblastoma cell lines, but not other tumors. These amplified sequences localize to homogeneously staining regions or double-minute chromosomes, suggesting a common function.

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    Area of Science:

    • Genetics
    • Oncology
    • Molecular Biology

    Background:

    • Neuroblastoma is a pediatric cancer with complex genetic alterations.
    • Amplification of specific DNA sequences is a common mechanism in cancer development.

    Purpose of the Study:

    • To identify and characterize DNA sequences specifically amplified in human neuroblastoma.
    • To investigate the chromosomal localization of these amplified sequences.

    Main Methods:

    • Southern blot analysis of EcoRI-digested human neuroblastoma DNA.
    • In situ hybridization with metaphase chromosomes.

    Main Results:

    • A family of discrete restriction fragments was found to be amplified in most neuroblastoma cell lines tested.

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  • These amplified sequences were not found in other human tumor types.
  • Localization to homogeneously staining regions or double-minute chromosomes in neuroblastoma cell lines.

    • Conclusions:

    • The identified DNA sequences are specifically amplified in neuroblastoma.
    • Amplified sequences in neuroblastoma cell lines share common features and may indicate a shared functional role.
    • Homogeneously staining regions and double-minute chromosomes in neuroblastoma may share common sequences and functions.