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A modified technique for combination of cytochemistry and radioautography of haemic cells
Scandinavian Journal of Haematology
|January 1, 1977
Abstract:
Cultured mouse bone marrow and human blood cells were labelled with 3H-thymidine, smeared, and stained, using peroxydase reagents or the May-Grünwald Giemsa procedure. The slides were dipped in a approximately 12% solution of polyvinylchloride, bleached by a photographic fixer and processed for liquid emulsion radioautography. This method yielded technically very satisfactory radioautographs of pre-stained smears.