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[Fluorometric method of determining urinary estrogens]
Abstract:
The authors describe a method for determination of estrogens in urine of nonpregnant women and men. The method includes enzymic hydrolysis of urine, gel filtration of Sefadex G25, extraction with organic solvents and modifies Kober reaction with spectrofluorometric determination. The technique of the method is not complex, the usage of reagents is small and is suitable for everyday work.