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[Fluorometric method of determining urinary estrogens].

T Stefanova, M Mutafchieva

    Eksperimentalna Meditsina I Morfologiia
    |January 1, 1978
    PubMed
    Summary

    This study presents a straightforward method for measuring estrogens in urine from nonpregnant women and men. The technique uses simple steps and minimal reagents, making it ideal for routine laboratory analysis.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry

    Context:

    • Estrogen determination is crucial for understanding various physiological processes.
    • Existing methods may be complex or require specialized equipment.

    Purpose:

    • To develop a simplified and efficient method for quantifying estrogens in human urine.
    • To provide a reliable technique suitable for routine laboratory use.

    Summary:

    • The described method involves enzymic hydrolysis of urine, Sefadex G25 gel filtration, organic solvent extraction, and a modified Kober reaction with spectrofluorometric detection.
    • This approach is characterized by its non-complex technique and minimal reagent consumption.

    Impact:

    • Enables more accessible and frequent monitoring of estrogen levels.
    • Supports research and clinical diagnostics by providing a practical analytical tool.

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