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Embryonic hemoglobins in mouse erythroleukemic cells
Research Communications in Chemical Pathology and Pharmacology
|December 1, 1983
Summary
T3C12 cells synthesize multiple hemoglobins, including A, E2, and E3, after induction. Embryonic mouse globins from yolk sac cells were also separated and characterized by their distinct electrophoretic mobilities.
Area of Science:
- Biochemistry
- Molecular Biology
- Hematology
Background:
- Murine erythroleukemic cells (T3C12) are a model for studying erythroid differentiation.
- Hemoglobin synthesis is a hallmark of erythropoiesis, involving the expression of specific globin chains.
Purpose of the Study:
- To characterize the hemoglobins synthesized by T3C12 cells upon induction with butyric acid and hemin.
- To analyze the composition and electrophoretic properties of embryonic mouse globins.
Main Methods:
- Isoelectric focusing (IEF) to determine isoelectric point (pI) values of hemoglobins.
- Acid urea triton x-100 electrophoresis to separate and analyze globin chains.
- Induction of T3C12 cells with butyric acid and hemin.
Main Results:
- T3C12 cells synthesize hemoglobins A, E2, and E3, as indicated by pI values and globin composition.
- Embryonic mouse globins (x, y, z, and alpha) from yolk sac cells were successfully separated.
- Electrophoretic mobility of embryonic globins followed the order: alpha > z > y > x.
Conclusions:
- Butyric acid and hemin induce the synthesis of specific adult hemoglobins in T3C12 cells.
- Distinct electrophoretic mobilities allow for the separation and identification of embryonic mouse globin chains.