Related Experiment Videos
A follow-up study of the development of Rauscher erythroleukemia
Abstract:
Expression of cell membrane antigens recognized by antiserum raised against purified Friend murine leukemia virus (Fr-MuLV) envelope glycoprotein (gp71) and by xenotropic MuLV-coded cell surface antigen (XenCSA) specific antibodies was studied in the course of the development of Rauscher erythroleukemia in the spleen of Balb/c mice. DNA content vs immunofluorescence or light scattering of cells were simultaneously analyzed. At early stages of the disease (4-5 days after infection) the gp71 and XenCSA-related antigen expression is enhanced mainly on S-G2/M-phase cells as compared to the majority of G1-phase cells or to the endogenous background of uninfected cells. Later (around 10 days after infection) an approximately ten-fold increased gp71-related antigen density is reached in every phase of the cell cycle. These data show that the virus-induced transition from resting to proliferating state is coupled to enhanced expression of both helper and defective viral env-gene products in the cell membrane of mitotic and G1-phase cells as well.
Insights
Friend murine leukemia virus (Fr-MuLV) infection enhances cell membrane antigen expression, particularly gp71 and XenCSA, during Rauscher erythroleukemia development in mice.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Rauscher erythroleukemia is a murine cancer model.
- Friend murine leukemia virus (Fr-MuLV) is an oncogenic retrovirus.
- Cell membrane antigens play roles in viral infection and cell cycle regulation.
Purpose of the Study:
- To investigate the expression patterns of Fr-MuLV envelope glycoprotein (gp71) and xenotropic MuLV-coded cell surface antigen (XenCSA) during Rauscher erythroleukemia progression.
- To correlate antigen expression with cell cycle phases in infected splenic cells.
Main Methods:
- Simultaneous analysis of DNA content, immunofluorescence, and light scattering in splenic cells from infected Balb/c mice.
- Utilized antisera against Fr-MuLV gp71 and XenCSA-specific antibodies.
Main Results:
- Early in disease (4-5 days post-infection), gp71 and XenCSA expression was elevated on S-G2/M-phase cells compared to G1-phase or uninfected cells.
- By 10 days post-infection, gp71-related antigen density increased approximately tenfold across all cell cycle phases.
- Enhanced antigen expression was observed on both helper and defective viral env-gene products.
Conclusions:
- Virus-induced transition from resting to proliferating states in Rauscher erythroleukemia is linked to increased expression of viral env-gene products.
- Both gp71 and XenCSA are upregulated on the cell membrane during erythroleukemia development.
- These viral antigens are expressed across all cell cycle phases as the disease progresses.