Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A vector for recombinant DNA in Staphylococcus aureus.

S Löfdahl, J E Sjöström, L Philipson

    Gene
    |April 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The gene for staphylococcal protein A.

    Immunology today·2014
    Same author

    Single cell assay with an automated capillary microinjection system.

    Cytotechnology·2012
    Same author

    Purification of B. megatherium Phage G and Evidence for a Muralytic Enzyme as an Integral Part of the Phage.

    The Journal of general physiology·2009
    Same author

    An outbreak of Salmonella Typhimurium infections in Denmark, Norway and Sweden, 2008.

    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2009
    Same author

    A large Escherichia coli O157 outbreak in Sweden associated with locally produced lettuce.

    Foodborne pathogens and disease·2008
    Same author

    The coxsackievirus and adenovirus receptor.

    Current topics in microbiology and immunology·2008
    Same journal

    Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

    Gene·2026
    Same journal

    Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

    Gene·2026
    Same journal

    Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

    Gene·2026
    Same journal

    Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

    Gene·2026
    Same journal

    Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

    Gene·2026
    Same journal

    Traumatic brain injury enhances fracture healing by upregulating VNN1 to activate the Wnt/β-catenin signaling pathway.

    Gene·2026
    See all related articles

    Researchers utilized staphylococcal plasmids to create recombinant DNA, successfully mapping the large staphylococcal plasmid pI258 using chloramphenicol resistance as a selection marker.

    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • Staphylococcal plasmids pS194 and pSC194 confer resistance to streptomycin and chloramphenicol.
    • These plasmids possess a single EcoRI restriction site, making them suitable for recombinant DNA construction.
    • Streptomycin resistance marker is inactivated by EcoRI cleavage, necessitating alternative selection methods.

    Purpose of the Study:

    • To construct hybrid plasmids by joining pSC194 with EcoRI fragments of staphylococcal plasmid pI258.
    • To develop a physical map for the large staphylococcal plasmid pI258.
    • To utilize chloramphenicol resistance as a positive selection marker for hybrid plasmid identification.

    Main Methods:

    • Employing staphylococcal plasmids pS194 and pSC194 as vectors.

    Related Experiment Videos

  • Utilizing the single EcoRI restriction site present in both vectors for DNA insertion.
  • Generating hybrid plasmids by ligating pSC194 with EcoRI-digested fragments of pI258.
  • Employing chloramphenicol resistance for the selection of successful recombinant DNA molecules.

    • Main Results:

    • Successful construction of hybrid plasmids containing pSC194 and fragments of pI258.
    • Demonstration that streptomycin resistance is not expressed in recombinant DNA due to EcoRI cleavage.
    • Establishment of a physical map for the staphylococcal plasmid pI258 based on the constructed hybrids.

    Conclusions:

    • Chloramphenicol resistance serves as an effective positive selection marker in staphylococcal plasmid-based recombinant DNA technology.
    • The study successfully elucidated the physical organization of the staphylococcal plasmid pI258.
    • The findings provide a foundation for further genetic manipulation and characterization of staphylococcal plasmids.