Antibodies against osteosarcoma and colon cancer

Two monoclonal antibodies with anti-human tumour reactivity were produced by murine hybridomas. One, 791T/36, is an IgG2b which was raised against a cultured human osteogenic sarcoma cell line, 791T. This antibody reacts with a high proportion of osteogenic sarcomas, and also with other tumour types. It has been used successfully as a tumour localising antibody in vivo and a targeting agent in vitro. The other antibody, C14/1/46, was raised against a human colonic adenoma. An IgM antibody, it reacts with most colorectal carcinoma cells but only with a small subpopulation of cells within the normal mucosa. It has been shown to recognise a difucosylated Type-2 blood-group chain, which has raised expression in colorectal carcinoma tissue.