Related Experiment Video
Updated: Aug 7, 2026

An Improved Method for the Preparation of Type I Collagen From Skin
Published on: January 22, 2014
Localization of human type II procollagen gene (COL2A1) to chromosome 12
Abstract:
DNA was prepared from 39 human-mouse somatic cell hybrid lines, and mouse and human parental cell lines. The DNA was digested to completion with EcoRI and Southern filters were prepared. These filters were hybridized at high stringency conditions to the human genomic subclone phHCol(II) A which corresponds to the human alpha 1(type II) procollagen gene (COL2A1). The mouse DNA yielded a single band at greater than 10 kb, whereas the human DNA had the expected single band at 4.8kb. Analyses of these human-mouse cell hybrids demonstrated that the human alpha 1(type II) procollagen gene segregates with chromosome 12.
Related Concept Videos
Karyotyping
Structural Protein Function
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Heterochromatin
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Collagens are the Major Structural Proteins of ECM
Connective tissue proper includes loose...
Fibril-associated Collagen
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Type IV Collagen of Basal Lamina
A type IV collagen molecule has six alpha chains which can exist in...

