Rapid and sensitive method for determination of haloperidol in human samples using nitrogen-phosphorus selective...

Area of Science:

• Pharmacology
• Analytical Chemistry
• Clinical Diagnostics

Background:

• Haloperidol is a crucial antipsychotic medication.
• Accurate monitoring of haloperidol plasma levels is essential for effective patient treatment.
• Existing analytical methods may lack the required sensitivity or specificity for routine clinical use.

Purpose of the Study:

• To develop and validate a sensitive and specific gas-chromatographic method for the quantitative analysis of haloperidol in human plasma.
• To establish a reliable method for determining haloperidol plasma concentrations in patients undergoing chronic treatment.

Main Methods:

• Gas chromatography with nitrogen-phosphorus selective detection.
• Use of azaperone as an internal reference standard.
• Analysis of haloperidol in human plasma samples from patients receiving doses ranging from 1.2 to 200 mg/day.

Main Results:

• The developed method demonstrates high sensitivity and specificity for haloperidol quantification.
• The method shows no interference from commonly used antipsychotic co-administered drugs.
• Accurate determination of haloperidol plasma levels is achievable within a wide therapeutic dosage range.

Conclusions:

• The described gas-chromatographic method is suitable for routine analysis of haloperidol plasma levels.
• This method facilitates precise therapeutic drug monitoring for psychotic patients on chronic haloperidol treatment.
• The technique's simplicity, specificity, and sensitivity enhance clinical management of haloperidol therapy.