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Organization and expression of genes encoding prostatic steroid binding protein
Annals of the New York Academy of Sciences
|January 1, 1984
Summary
Testosterone regulates prostatic steroid binding protein gene expression. While testosterone stimulates C3 gene transcription, it may act post-transcriptionally in mouse cells, not directly on the promoter.
Area of Science:
- Molecular biology
- Genetics
- Endocrinology
Background:
- Prostatic steroid binding protein (PSBP) gene expression is regulated by testosterone.
- Two C3 genes exist, C3(1) and C3(2), with differential DNA methylation and in vivo transcription.
Purpose of the Study:
- To investigate the mechanism of testosterone-regulated PSBP gene expression.
- To determine if testosterone interacts directly with the C3 promoter.
Main Methods:
- Cloning and introduction of PSBP genes into mouse S115 cells.
- Construction of chimeric genes with C3 promoters and a marker gene (interferon).
- Analysis of gene transcription and hormonal stimulation.
Main Results:
- Both C3 genes were transcribed in mouse cells and stimulated by testosterone.
- Testosterone stimulation of chimeric gene expression was less than twofold.
- Testosterone's effect may occur post-transcriptionally in some clones.
Conclusions:
- Testosterone does not directly interact with the rat C3 promoter in mouse cells.
- Hormonal regulation of C3 gene expression can occur post-transcriptionally.