Human osteogenic sarcoma: fine structure of the fibroblastic type

The fine structure of representative regions of nine fibroblastic osteogenic sarcomas was studied. As judged by light microscopic criteria, the tumors represented both highly malignant (grades 3-4) and less malignant (grade 2) varieties. By electron microscopy, six basic cell types were found in the selected regions (fibroblastlike, histiocytelike, and myofibroblastlike cells, along with xanthoma cells, multinucleated giant cells, and undifferentiated cells). In addition, occasional osteoblastlike cells were encountered. Fibroblastlike cells in general, and especially in grade 2 tumors, showed a fine structure, enabling differentiation from osteoblastlike cells. Multinucleated giant cells were of two morphologic types, and the fine structure appeared to be related to the malignancy potential and differentiation of the tumors. Many multinucleated giant cells in grade 2 tumors had a ruffled border and appeared to be highly active in digestive events (especially phagocytosis of whole cells and portions of cells). Accumulation of variable amounts of lipid in droplet form was common in the various types of cells present in the tissues. The observations were discussed with particular emphasis on the interrelationships and functional roles of the cells.