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Can alpha-1-protease inhibitor be used in replacement therapy?
The American Review of Respiratory Disease
|February 1, 1983
Summary
Alpha-1-protease inhibitor (alpha 1 PI) shows promise for preventing emphysema in deficient individuals and treating acute respiratory distress syndrome (ARDS). A novel purification method allows for its effective isolation and potential clinical application.
Area of Science:
- Biochemistry
- Pulmonology
- Protein Chemistry
Background:
- Alpha-1-protease inhibitor (alpha 1 PI) deficiency is linked to emphysema.
- Adult respiratory distress syndrome (ARDS) is a critical condition requiring effective treatments.
Purpose of the Study:
- To explore the therapeutic potential of alpha 1 PI in emphysema and ARDS.
- To develop an effective method for isolating alpha 1 PI from plasma fractions.
Main Methods:
- Utilized a unique disulfide bond in alpha 1 PI for selective separation.
- Employed reductive-salting out combined with DEAE-anion exchange chromatography.
- Investigated pasteurization of purified alpha 1 PI with stabilizing additives.
Main Results:
- Successfully isolated alpha 1 PI from Cohn Fraction IV-1.
- Demonstrated that alpha 1 PI's inhibitory activity and stability are preserved after reduction.
- Confirmed that the purified alpha 1 PI can be effectively pasteurized.
Conclusions:
- A viable method for isolating therapeutic-grade alpha 1 PI has been established.
- The findings support further clinical investigation for emphysema and ARDS.
- This purification technique utilizes a side product of albumin production, enhancing resource utilization.