In vitro sterilization of T lymphocyte lines infected with bacteria

Rat T lymphocyte lines in culture contaminated with bacteria can be sterilized by a simple manoeuvre. The infected T cells are crudely separated from the bulk of contaminating organisms in an albumin density gradient. They are completely sterilized after overnight culture in medium containing 20% fresh serum from a conventionally kept adult rat in the presence of irradiated syngeneic adherent spleen cells.