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Harvest of human bone marrow directly from bone
Journal of Immunological Methods
|April 27, 1984
Summary
Directly harvesting human bone marrow from resected bone yields purer cell preparations than traditional methods. Vertebrae are the optimal source for this high-quality bone marrow, crucial for transplantation and research.
Area of Science:
- Immunology
- Hematology
- Transplantation Biology
Background:
- Conventional bone marrow aspiration can contaminate samples with peripheral blood lymphocytes.
- Mature T cells in bone marrow grafts may impact allogeneic transplantation outcomes.
Purpose of the Study:
- To identify the optimal source of human bone marrow for direct harvesting.
- To evaluate the purity and cellular composition of directly harvested bone marrow.
Main Methods:
- Human bone marrow was harvested from surgical specimens and cadaveric donors.
- Marrow yield was compared from vertebrae, ilia, and ribs.
- Density gradient centrifugation was used for mononuclear cell isolation.
Main Results:
- Vertebral bodies yielded the highest average cell count (3.1 x 10^9 cells).
- Directly harvested marrow showed lower peripheral blood lymphocyte contamination.
- Isolated T cells demonstrated in vitro immunological competence.
Conclusions:
- Direct bone marrow harvesting, particularly from vertebrae, provides superior cell purity.
- This method is promising for obtaining human bone marrow for immunological studies and allogeneic transplantation.