[Rapid method of determining beta-lactamase inhibitor activity]

Area of Science:

• Biochemistry
• Enzymology
• Analytical Chemistry

Background:

• Beta-lactamase enzymes hydrolyze beta-lactam antibiotics, contributing to antibiotic resistance.
• Accurate and rapid determination of beta-lactamase activity is crucial for research and development.

Purpose of the Study:

• To describe a simple and rapid spectrophotometric method for quantifying beta-lactamase activity.
• To validate the method for quality control applications.

Main Methods:

• Enzymatic assay utilizing spectrophotometry.
• Benzylpenicillin as the substrate for beta-lactamase activity determination.
• Monitoring the spectrophotometric changes associated with benzylpenicillin hydrolysis.

Main Results:

• The described method provides a straightforward and fast determination of beta-lactamase activity.
• The spectrophotometric approach allows for real-time monitoring of enzymatic reactions.

Conclusions:

• The developed method is suitable for the rapid assessment of beta-lactamase activity.
• This technique is recommended for the quality control of beta-lactamase inhibitor production.