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Related Experiment Videos

A "column-batch" method for separating MB and LD1 in a single fraction.

L G Morin, E G Barton

    Clinical Chemistry
    |October 1, 1983
    PubMed
    Summary
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    This study introduces a column-batch method for creatine kinase (CK) isoenzyme separation, offering concentrated fractions for accurate automated analysis without MM isoenzyme interference. The method efficiently isolates MB and BB isoenzymes or a combined MB, BB, and LD1 fraction.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Accurate quantification of creatine kinase (CK) and lactate dehydrogenase (LD) isoenzymes is crucial for diagnosing myocardial infarction.
    • Existing ion-exchange methods often result in diluted fractions, requiring modifications for conventional automated assays.
    • Interference from the abundant MM isoenzyme of CK can complicate analysis.

    Purpose of the Study:

    • To develop and validate a "column-batch" method for the efficient separation of CK isoenzymes (MB, BB) and LD1 isoenzyme.
    • To achieve concentrated eluent fractions for simplified automated activity measurements.
    • To eliminate interference from the MM isoenzyme of CK during analysis.

    Main Methods:

    • Utilized a strong anion exchanger (AG-MP1, Bio-Rad) in acetate form at pH 6.3.

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  • Employed a column-batch technique allowing for either separation of MB from BB or combined isolation of MB, BB, and LD1.
  • Eluted retained isoenzymes (MB, BB, LD1) using a magnesium acetate solution, while MM isoenzyme was not retained.

    • Main Results:

    • The developed method yields twofold concentrated fractions compared to the applied sample.
    • No retention of the MM isoenzyme was observed, preventing interference.
    • Separated fractions containing MB, BB, and LD1 were obtained, suitable for conventional automated assays without modifications.
    • Results correlated well with immunoinhibition methods for subunit B and LD1.

    Conclusions:

    • The column-batch method provides an efficient and accurate approach for CK and LD isoenzyme separation.
    • Concentrated fractions simplify diagnostic procedures by enabling direct use with automated analyzers.
    • The method's robustness against MM isoenzyme interference enhances its clinical utility for myocardial infarction diagnosis.