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A differential amplifier circuit for reducing noise in axial light loss measurements
Abstract:
Simplified electronics have been developed for reducing laser excitation source light intensity fluctuations in flow cytometric axial light loss (extinction) measurements. By continuously monitoring the laser output with a photodiode detector and combining it with the axial light loss signal from a second detector using a high-speed differential amplifier, background interference is reduced 10 to 40 dB. Oscilloscope waveforms and frequency distribution histograms recorded from uniform-size polystyrene latex spheres and human mononuclear blood cells are used to illustrate the noise reduction capabilities.
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