Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ascorbic acid and alkaline phosphatase activity.

G A Miggiano, A Mordente, G E Martorana

    Enzyme
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Comparative species distribution model framework for marine conservation and its application to loggerhead turtles in the Mediterranean.

    Conservation biology : the journal of the Society for Conservation Biology·2026
    Same author

    Spatio-temporal dynamics of floating marine macro-litter in Italian waters (2018-2023): The impact of the COVID-19 pandemic.

    Environmental pollution (Barking, Essex : 1987)·2026
    Same author

    From juveniles to adults: Ontogenetic and seasonal patterns of loggerhead turtles in oceanic Mediterranean habitats.

    Marine environmental research·2026
    Same author

    Non-thyroidal illness syndrome in chronic diseases: role of irisin as modulator of antioxidants.

    European review for medical and pharmacological sciences·2023
    Same author

    Vespa velutina nigrithorax venom allergy: inhibition studies approach for the choice of specific immunotherapy.

    European annals of allergy and clinical immunology·2022
    Same author

    Review of decontamination protocols for shared non-critical objects in 35 policies of UK NHS acute care organizations.

    The Journal of hospital infection·2021
    Same journal

    More on effects of storage time and temperature on urinary enzymes: a 1-year study.

    Enzyme·1992
    Same journal

    Automated measurement of lactate dehydrogenase, alkaline phosphatase and gamma-glutamyltransferase in urines: an alternative to the manual procedure.

    Enzyme·1992
    Same journal

    Hepatocyte heterogeneity in the metabolism of fatty acids: discrepancies on zonation of acetyl-CoA carboxylase.

    Enzyme·1992
    Same journal

    Characterization of multiple forms of carbonyl reductase from chicken liver.

    Enzyme·1992
    Same journal

    Identification of tyrosylprotein sulfotransferase in rat gastric mucosa.

    Enzyme·1992
    Same journal

    Essential fructosuria: increased levels of fructose 3-phosphate in erythrocytes.

    Enzyme·1992
    See all related articles

    Ascorbic acid significantly inhibits bovine kidney alkaline phosphatase activity in laboratory settings. Enzyme activity reduction depends on vitamin concentration, time, and temperature, but is lessened by the substrate.

    Area of Science:

    • Biochemistry
    • Enzymology

    Background:

    • Alkaline phosphatase is a crucial enzyme in various biological processes.
    • Understanding enzyme regulation is vital for biochemical research.

    Purpose of the Study:

    • To investigate the in vitro effect of ascorbic acid on bovine kidney alkaline phosphatase activity.
    • To characterize the inhibitory mechanism of ascorbic acid on alkaline phosphatase.

    Main Methods:

    • Enzyme activity assays were performed in vitro.
    • Ascorbic acid concentration, incubation time, and temperature were varied.
    • The effect of the substrate on enzyme inhibition was assessed.

    Main Results:

    • Ascorbic acid markedly decreased bovine kidney alkaline phosphatase activity.

    Related Experiment Videos

  • The inhibition was dependent on ascorbic acid concentration, time, and temperature.
  • Enzyme activity inhibition was reduced in the presence of the substrate.

    • Conclusions:

    • Ascorbic acid acts as a potent inhibitor of bovine kidney alkaline phosphatase in vitro.
    • The inhibitory effect is modulated by reaction conditions and substrate presence.
    • These findings contribute to understanding enzyme kinetics and vitamin interactions.