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Dental lamina develops even within the mouse diastema.
Journal of Craniofacial Genetics and Developmental Biology
|January 1, 1983
Summary
The epithelial lining in mouse upper jaw diastemas forms a temporary tooth rudiment, indicating diastemas develop secondarily through regression. Incomplete regression is observed in some mouse mutants.
Area of Science:
- Developmental Biology
- Oral and Maxillofacial Surgery
- Genetics
Background:
- The development of the mammalian jaw, specifically the upper maxillary diastema, is crucial for understanding craniofacial morphogenesis.
- Previous research has not fully elucidated the early developmental origins and potential odontogenic capacity of the diastema epithelium.
Purpose of the Study:
- To investigate the presence and fate of epithelial rudiments within the developing upper maxillary diastema of ICR strain mouse embryos.
- To determine if the diastema originates de novo or through secondary regression of a pre-existing structure.
- To assess the implications of diastema tissue's odontogenic potential for future research.
Main Methods:
- Histological examination of frontal sections from mouse embryos (ICR strain) at specific developmental stages (days 13-15).
- Staining techniques included hematoxylin-eosin and Periodic Acid-Schiff (PAS) to visualize epithelial structures.
- Analysis focused on the epithelial lining of the future upper maxillary diastema and its relationship with dental lamina and tooth anlagen.
Main Results:
- A continuous epithelial rudiment, resembling dental lamina, was identified in the anterior extension of the first upper molar anlage up to the primary choana by day 13.
- A distinct tooth anlage (bud stage) was observed, which later fragmented.
- Beginning on day 14, this epithelial rudiment showed regression, persisting only posteriorly by day 15, merging with the first upper molar's dental lamina.
Conclusions:
- The presence of a transient dentition rudiment in the embryonic upper maxillary diastema provides ontogenetic evidence that the diastema forms secondarily through regression.
- In certain mouse mutants (tabby, crooked, sleek), this regression process appears incomplete, suggesting a genetic influence on diastema formation.
- The inherent odontogenic potential of diastema tissues must be considered in in vitro studies of tooth development and inductive interactions.