Cholinoceptive properties of respiratory neurones in the rat medulla

The effects of iontophoretically applied acetylcholine, the acetylcholine agonists nicotine and muscarine, and the antagonists atropine, dihydro-beta-erythroidine (DH beta E) and mecamylamine, together with the excitatory amino acids, glutamate and D,L-homocysteic acid (DLH) were examined on the activity of respiratory-related neurones in the rat medulla and were compared with effects on non-respiratory brain stem neurones. Most neurones were excited by acetylcholine and no inhibitory responses were seen. Glutamate and DLH also excited but there was a trend for the phasic activity of respiratory neurones to be converted to a tonic discharge. Nicotine also excited most neurones to which it was applied and these responses were blocked by DH beta E but not by atropine. Muscarine also caused excitation and these responses were blocked by atropine but not by DH beta E. Both antagonists blocked acetylcholine-induced excitation but had no effect on responses to glutamate or DLH. Mecamylamine was without effect. It is concluded that the proportion of cholinoceptive respiratory neurones in the rat brain stem is similar to that for non-respiratory neurones. It seems likely that both nicotinic and muscarinic receptors are present on the majority of respiratory neurones and that both contribute to the response produced by iontophoretically-applied acetylcholine.