Amino acid sequence of rat liver thiol proteinase inhibitor

The complete amino acid sequence of rat liver thiol proteinase inhibitor is presented. Its unique 98 residue sequence was derived mostly from analyses of a limited proteolysis product generated during the isolation of the protein with an active papain affinity column and of fragments produced by cleavage at arginyl bonds. The intact protein prepared with a tetrathionate inactivated ficin column has a unique amino-terminal sequence (Ac-Met-Met-Cys-). The inhibitor has no sequence homology with any proteinase inhibitor of known structure.