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Related Experiment Videos

Light scattering measurements supporting helical structures for chromatin in solution.

A M Campbell, R I Cotter, J F Pardon

    Nucleic Acids Research
    |May 1, 1978
    PubMed
    Summary

    Chromatin structure, specifically polynucleosomes, forms a loosely helical shape at low ionic strength. This helix becomes more regular with increasing ionic strength, aligning with the solenoid model.

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    Area of Science:

    • Biophysics
    • Molecular Biology
    • Structural Biology

    Background:

    • Understanding chromatin structure is crucial for gene regulation.
    • Polynucleosomes represent higher-order chromatin structures.

    Purpose of the Study:

    • To investigate the structural organization of polynucleosomes.
    • To determine how ionic strength affects chromatin folding.

    Main Methods:

    • Laser light scattering measurements.
    • Determination of radii of gyration.
    • Analysis of polynucleosomes (50-150 nucleosomes) across varying ionic strengths.

    Main Results:

    • At low ionic strength, chromatin adopts a loosely helical structure.

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  • Increasing ionic strength leads to a more regular helical conformation.
  • Observed dimensions are consistent with the Finch and Klug solenoid model.

    • Conclusions:

    • Ionic strength is a key factor in determining higher-order chromatin structure.
    • Polynucleosomes exhibit a transition from a loose helix to a more defined solenoid structure.
    • These findings support existing models of chromatin organization.