Palmitate incorporation into different brain regions in the awake rat

A quantitative method is presented to examine palmitate flux into a stable metabolic compartment in individual brain regions of awake rats. Following the i.v. injection of [14C]palmitate, brain radioactivity rose and then fell until, at 4 h, a stable concentration was reached that was maintained for up to 24 h. The flux of plasma palmitate into this 4 h compartment was calculated by dividing regional brain radioactivity at 4 h, as determined by quantitative autoradiography, by the integral of the plasma palmitate specific activity. Palmitate flux varied from 2.0 x 10(-5) mumol/g.s into the internal capsule to 9.3 x 10(-5) mumol/g.s into the arcuate nucleus, and generally was proportional to the regional cerebral metabolic rate for glucose, as measured with 2-deoxy-D-[1-14C]glucose. The results demonstrate that it is possible to determine unidirectional palmitate flux into a stable metabolic compartment in individual brain regions of awake rats, that flux into gray matter regions generally exceeds flux into white matter, and that palmitate flux is proportional to published values for regional brain oxidative metabolism.