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Atomic absorption determination of serum copper: collaborative study.

D L Osheim

    Journal - Association of Official Analytical Chemists
    |September 1, 1983
    PubMed
    Summary

    A collaborative study validated a serum copper assay using Atomic Absorption Spectroscopy (AAS). This method demonstrated reliable intralaboratory and interlaboratory precision, leading to its official adoption.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Serum copper analysis is crucial for diagnosing various medical conditions.
    • Interlaboratory variations in serum copper measurements necessitate method standardization.

    Purpose of the Study:

    • To evaluate the performance of a specific Atomic Absorption Spectroscopy (AAS) method for serum copper determination.
    • To assess the reliability and reproducibility of the chosen AAS method across multiple laboratories.

    Main Methods:

    • A survey of laboratory methods preceded a collaborative study.
    • Twelve laboratories analyzed bovine serum samples using a standardized AAS method.
    • Serum samples were diluted 1:1 with distilled water; standards were diluted with 10% glycerin.

    Main Results:

    • Intralaboratory coefficients of variation (CV) ranged from 2.24% to 4.40%.
    • Interlaboratory CV ranged from 2.56% to 6.05%.
    • The method showed acceptable precision for serum copper analysis.

    Conclusions:

    • The validated AAS method for serum copper determination is precise and reproducible.
    • The method has been adopted as an official first action method, indicating its suitability for routine use.

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