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Related Experiment Videos

How phagocytic leukocytes move.

J H Hartwig, H L Yin, T P Stossel

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |September 1, 1983
    PubMed
    Summary
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    Phagocyte function relies on coordinated cytoplasmic movement, regulated by actin, myosin, and gelsolin. Calcium ions (Ca2+) activate gelsolin, controlling cytoplasmic gel-to-sol transitions essential for cell response.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Immunology

    Background:

    • Phagocyte function requires regulated cytoplasmic movement, powered by actin and myosin.
    • Actin filaments in the cell cortex can exist as a fluid or form a gel network.

    Purpose of the Study:

    • To elucidate the molecular mechanisms regulating cytoplasmic movement in phagocytes.
    • To understand the role of gelsolin and calcium ions (Ca2+) in controlling cytoplasmic dynamics.

    Main Methods:

    • Investigated the role of actin-binding proteins in forming cytoplasmic gel networks.
    • Examined the regulatory function of gelsolin in response to calcium ion (Ca2+) concentrations.
    • Studied the reversible transformation of cytoplasm between gel (sol) and sol (gel) states.

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    Main Results:

    • Gelsolin, activated by micromolar calcium (Ca2+), causes actin filament shortening and gel network disintegration.
    • The cytoplasmic gel network reforms when calcium (Ca2+) levels decrease below the gelsolin activation threshold.
    • Calcium ions (Ca2+), via gelsolin, regulate the sequence of events initiated by phagocyte plasma membrane stimulation.

    Conclusions:

    • Cytoplasmic movement regulation is crucial for phagocyte function.
    • Gelsolin and calcium ions (Ca2+) are key regulators of cytoplasmic dynamics, controlling actin-based motility.
    • Understanding these mechanisms may reveal factors affecting leukocyte function.