Re-examination of the DNA-mediated transformation in goldfish

DNAs were isolated from goldfish, and the balancer bearing species of newt. They were injected separately into fertilized goldfish eggs. A balancer developed in some newt-DNA injected goldfish. DNA injected goldfish eggs occasionally developed a non-balancer process. These results demonstrated the newt DNA-mediated transfer of the species character from newt to goldfish. The injected DNA was localized by the method of autoradiography using H3-cDNA. Silver grains were found in areas over the colchicine induced metaphase. The incorporation of foreign DNA into chromosomes provides a material basis for genetic transformation.