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Updated: Aug 10, 2026
Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
Published on: September 3, 2014
Major differences in the concanavalin A binding glycoproteins of postsynaptic densities from rat forebrain and
Abstract:
A highly purified fraction of postsynaptic densities (PSDs) was isolated from rat forebrain and cerebellum. The glycoproteins in the PSDs from the two brain regions were compared by probing nitrocellulose blots with concanavalin A. This approach revealed the presence of 8, previously undetected PSD glycoproteins and a 230K M, glycoprotein unique to forebrain PSDs and 260K Mr glycoprotein unique to cerebellar PSDs. There were also variations in the relative abundances of glycoproteins common to both brain regions. These observations may indicate that individual PSDs differ in their complement of major glycoproteins.
